Assay Automation & Detection ®
Decision Biomarker's AVANTRA ® biomarker workstation integrates our proprietary technology breakthroughs in microarray surface chemistry for protein binding, assay automation and detection optics for microarray imaging. This results in fully automated, multiplex immunoassays without the need for robotic handling, and dramatically reduces instrument complexity, labor, and assay cost and time.
Capture antibodies are spotted on our PATH® Protein Microarray Substrate, thin-film (<1 micron thick) nitrocellulose 2-D film. This unique surface dramatically reduces background fluorescence while minimizing non- specific binding. These features are extremely important when multiplexing several analytes in one experiment.
Key to the system's performance is our proprietary reagent-handling system that stores detection antibodies and reagents in our biochip and automatically releases them during the assay processing time.
After the automated multiplex ELISA has been completed (average run time of less than 45 minutes), the entire image of the biochip surface is detected by the system's CCD camera. Results are displayed and are calculated using the instrument's software.
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How It Works
The AVANTRA ® biomarker workstation takes advantage of the latest in optical component technologies that deliver higher performance at lower cost. LEDs that deliver approximately 200mW of excitation power; excitation and emission filters to support the use of Cyanine 3 detection; and custom lenses to capture assay images and transmit them to a cooled CCD imager.
Capture antibodies are spotted on our PATH® Protein Microarray Substrate, thin-film (<1 micron thick) nitrocellulose 2-D film. This unique surface dramatically reduces background fluorescence while minimizing non- specific binding. These features are extremely important when multiplexing several analytes in one experiment.
Key to the system's performance is our proprietary reagent-handling system that stores detection antibodies and reagents in our biochip and automatically releases them during the assay processing time.
After the automated multiplex ELISA has been completed (average run time of less than 45 minutes), the entire image of the biochip surface is detected by the system's CCD camera. Results are displayed and are calculated using the instrument's software.
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